RBS

Part:BBa_B0071

Designed by: Barry Canton   Group: iGEM04_MIT   (2006-03-03)


Specialized RBS

This RBS has the modified Shine-Dalgarno sequence, it has a reduced secondary structure relative to using the actual Brink sequence with mixed connective sites flanking it. This RBS is modified from B0071 by reducing the 3' sequence so that the SD to ATG spacing is closer to that which is optimal for E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//rbs/prokaryote/constitutive/miscellaneous
//ribosome/prokaryote/custom
//chassis/prokaryote/ecoli
//direction/forward
//regulation/constitutive
Parameters
None